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KMID : 0988420030150010015
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Journal of Dental Science (C.N.U.)
2003 Volume.15 No. 1 p.15 ~ p.25
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Evaluation of Cytotoxicity of Electroplated stainless Steel Orthodontic Wire
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Abstract
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The purpose of this study was to evaluate the possible clinical application through examining the cytotoxicity of orthodontic wire, which was increased diameter using electroplating.
First, nickel plating was done on the commercially available stainless steel wire using a electroplating technique. And for the comparison electroplated wire with ready made stainless steel wire and titanium or copper. each wire was incubates for 72 hours on the medium. The release of the metal ion was measured using ICP-AES. The incubated medium was diluted 75%, 50%, 25% and added on the microplate each with Balb/c 373 mouse fibroblast for MTT analysis and compared with medium only. Through the measuring of the change in absorbency value of each wire group and the difference of absorbency value according to change of dilution, following results were obtained.
1. The results of ICP-AES analysis showed that lots of nickel ions were isolated from electroplated orthodontic wires and lots of copper ions were isolated from copper.
2. The inverted microscope showed that numbers of cells were decreased at the electroplated wire and copper.
3. The results of MTT analysis showed that there was no difference of the absorbency value of titanium at any dilution.
4. The results of MTT analysis showed that the absorbency values of the electroplated wires were lower than those of medium only at all dilutions, and showed statistically significant differences(p<0.001).
5. The results of MTT analysis showed that the absorbency value of the stainless steel wires were lower than those of medium only at all dilutions, and showed statistically significant differences(p<0.05).
6. The results of MTT analysis showed that the absorbency value of the copper were lower than those of medium only at all dilutions, and showed statistically significant differences(p<0.001).
7. Assessment as per ISO 10993, part 5, titanium and stainless steel wire investigated corresponded to "non-cytotoxic". Whereas copper was "highly cytotoxic", electroplated wire was alloted to "moderate cytotoxic".
The results of this study indicate that the electroplated orthodontic wires need additional efforts in order to decrease cytotoxicity for their clinical applications.
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